Extracellular vesicle microRNA cargoes from intermittent hypoxia-exposed cardiomyocytes and their effect on endothelium

Intermittent hypoxia (IH), a main characteristic of obstructive sleep apnea (OSA) syndrome, is an independent risk factor cardiovascular complications. However, the mechanism has not been fully elucidated. Growing evidence revealed alterations extracellular vesicle (EV) contents, mostly miRNAs, playing pathogenic role in In current study, we attempt to compare disparity myocardial EV miRNA components after IH or normoxia treatment and determine whether EVs from IH-treated cardiomyocytes could affect endothelial function. 63 differentially expressed miRNAs were identified IH-exposed by chip assay. Among them, 16 with homologous sequence mouse human verified qPCR assay 11 proved same tendency as KEGG predicted that function was enriched Akt signaling pathway. Notably, dramatically impaired endothelial-dependent relaxation inhibited Akt/eNOS expression cells. This study provides first significantly alters composition reveals novel under status, which will help understand OSA- IH-related dysfunction new scope. • are validated different EVs. signal pathway miRNAs. impair inhibit expression.